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. 2015 Dec 3;44(6):e51. doi: 10.1093/nar/gkv1327

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Quantitative analysis K_d's. Affinity measurements between ATF1 (A), c-Jun (B), c-Fos (C), AP-1 (D) and CRE sequences. We programmed the QPID device with different CRE consensus sequences. Each TF was immobilized to the device surface, flooding DNA chambers solubilized spotted DNA, allowing TF and DNA to interact. Protein expression levels (Cy3) and interacting DNA signals (Cy5) were measured. Interaction ratio – Cy5\Cy3 (colors refer to oligonucleotide number in Figure 3).