Table 2. K_d values (μM) for binding of ATF1, c-Jun, c-Fos and AP-1 TFs to different CRE consensus sequences.

Oligo #	BS	ATF1	c-Jun	c-Fos	c-Fos/c-Jun
1	CRE	0.04	1.3	-	0.19
2	CRE	0.03	2.4	-	0.15
3	AP-1	11	2.5	-	0.27
4	AP-1	17	-	-	0.50
5	HS	0.17	-	-	3.5
6	HS	0.04	-	-	4.0
7	Mutant	-	-	-	-

Results were fitted using nonlinear least square minimization. QPID's sensitivity is limited to 50 μM for quantitative affinity measurement. Weaker interactions were marked as ‘-’.