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. 2015 Dec 10;44(6):e54. doi: 10.1093/nar/gkv1338

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Schematic representation of pAAV-2Aneo. (A) A plasmid map of pAAV-2Aneo. ITR: inverted terminal repeat, MCS: multiple cloning site, IVS: synthetic intron, 2A: Thosea asigna virus 2A, Neo^R: neomycin resistance (neomycin phosphotransferase) gene, pA: polyadenylation site, f1 ori: fi single-strand DNA origin, Amp^R: ampicillin resistance (β-lactamase) gene, pUCori: pUC plasmid replication origin. (B and C) Diagrams of targeting vectors constructed based on pAAV-2Aneo in which a promoter-trap module is inserted into an intron (B) or an exon (C). Predicted splicing of mRNAs derived from these targeting vectors is shown at the bottom of the diagrams. HA: homology arm.