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. 2015 Dec 10;44(6):e54. doi: 10.1093/nar/gkv1338

Figure 3.

Figure 3.

H/R ratios of AAV-based targeting vectors assessed by disruption of PIGA exon 6. (A) A scheme describing the disruption of PIGA exon 6. Thin dotted lines indicate homologous regions between the wild-type PIGA locus and targeting vectors. Stop: artificially introduced stop codon (TAA). For other abbreviations, see legends for Figures 1 and 2. (B) H/R ratios of targeting vectors disrupting PIGA exon 6. Five cell lines denoted on the left were infected with the AAV-based targeting vector PIGAex6-TV-IRES (IRES), PIGAex6-TV-2A (2A) or an AAV vector targeting an unrelated gene created based on pAAV-2Aneo (VC). Infected cells were selected with G418, stained with FLAER, and then analyzed by FCM. Cells infected with VC but not stained with FLAER were included as further controls. Shown are representative dot plots with the ratio of FLAER-negative cells noted within respective plots. (C) Graphical representation of data obtained with cells subjected to FLAER-staining in the assay described in (B) (mean ± s.e.m.; n = 3).