Figure 3.

In vivo study of the synergistic and antagonistic dual-input genetic circuits. Mice were co-transfected with 100 μg of pT6U3-Fluc, 10 μg of pGAVPO and 10 μg of prTetR-VP16 (A) or 100 μg of pTetR-KRAB (C) via a hydrodynamic procedure. The mice were then fed in cages with glass bottoms in darkness without (Dark Dox-) or with (Dark Dox+) Dox, or in 90 mW·cm⁻² blue light irradiance without (Light Dox-) or with (Light Dox+) Dox. Mice got injection of only Ringer's solution without any plasmids and kept in darkness without Dox addition were used as the control. In situ imaging of firefly luminescence was carried out 12 h after intravenous injection of plasmids by tail intravenous injection of D-luciferin (150 μg/g of body weight, i.p.) under ether anesthesia. (B) and (D), a region of interest (ROI) was drawn around each liver location, and the number of photons/second was calculated. The data are shown as the mean ± SD (n = 3–4).