Table 1. Length of integration of donor DNA.

Donor DNAa	Divergence	Left end (in bp)		Right end (in bp)		Integration	Observed divergence
	(in %)	recipient	donor	donor	recipient	(in bp)	(in %)
Bsu W23	2.47b	1	350	2501	2567	≈2359	2.5
		1	350	2294	2567	≈2256	2.9
		395	605	2642	2651	≈2147	3.0
		395	605	2690	2918	≈2304	3.0
		395	605	2924	2948	≈2436	2.9
Bat 1942	8.35	479	608	1508	1590	≈1006	8.9
		479	608	1590	1598	≈1060	8.9
		932	941	2133	2165	≈1213	10.0
		920	932	1733	1736	≈ 809	8.8
		878	896	1463	1464	≈ 577	9.7
Bam DSM7	10.12	1172	1184	1535	1590	≈ 385	10.6
		1226	1238	1535	1590	≈ 385	10.0
		1175	1214	1508	1590	≈ 355	8.2
		1233	1245	1535	1590	≈ 324	10.0
		1250	1277	1535	1590	≈ 299	10.4
Bli DSM13	14.52	1184	1190	1577	1589	≈ 270	15.6
		1328	1334	1451	1463	≈ 252	13.1
		1211	1224	1451	1463	≈ 240	15.0
		1328	1334	1556	1565	≈ 230	13.0
		1328	1334	1493	1508	≈ 170	14.1
Bth MC28	20.83	1439	1443	1443	1445	≈ 3	33.3
		1439	1443	1443	1445	≈ 3	33.3
		1439	1443	1443	1445	≈ 3	33.3
		1442	1443	1443	1448	≈ 3	33.3
		1439	1443	1451	1452	≈ 9	22.2

^aThe length of donor DNA is 2997 bp and the Rif^R mutation is located at position 1443.

^bThe first mismatch is at position 350 of the donor DNA (see Materials and methods).