Table 2. Dissociation constants (Kd,BP and Kd,ZP2) for B-DNA and Z-DNA binding, equilibrium constant for B–Z transition (KBZ,1) and association/dissociation rate constants (kon,ZP and koff,ZP2) for Z-DNA binding of caZαPKZ with a 6-bp DNA.
| DNA | pH | [NaCl] (mM) | Kd,BP (μM) | Kd,ZP2 (μM) | KBZ,1 | kon,ZP (×108 M−1s−1) | koff,ZP2 (s−1) |
|---|---|---|---|---|---|---|---|
| dT(GC)3 | 6.0 | 10 | 0.028 ± 0.017 | 0.345 ± 0.079 | 0.87 ± 0.03 | 19.6 ± 1.2a | 675 ± 43a |
| 100 | 16.4 ± 0.8 | 8.76 ± 0.67 | 0.19 ± 0.02 | 1.56 ± 0.03b | 1381 ± 30b | ||
| 8.0 | 10 | 0.157 ± 0.021 | 0.129 ± 0.074 | 1.18 ± 0.03 | |||
| 100 | 5.41 ± 0.66 | 2.41 ± 0.37 | 0.18 ± 0.02 | ||||
| d(GC)3 | 8.0 | 10 | 5.18 ± 2.43 | 1.79 ± 0.95 | 0.11 ± 0.05 |
aFrom CPMG data with Pfree = 0.873.
bFrom CPMG data with Pfree = 0.891.