Skip to main content
. 2016 Jan 28;44(6):2888–2897. doi: 10.1093/nar/gkw041

Figure 2.

Figure 2.

Expression of the cold-shock protein RBM3 is altered between 37°C and 40°C in THP-1-derived macrophages. (A) Confirmation of THP-1 differentiation into macrophages as shown by increased expression of CD11b and CD44 using flow cytometry. (B) Viability of THP-1 cells incubated at 40°C compared to controls (37°C) as measured by flow cytometry following Annexin V and propidium iodide (PI) staining. Ultraviolet-exposed cells (UV+) were used as positive control. (C) Gene function enrichment of genes overexpressed at 40°C. (D) Top ten most downregulated genes at 40°C. (E) Western blot for RBM3 expression following incubation at 37°C and 40°C with actin used as the loading control. PMA, phorbol-12-myristate 13-acetate.