Figure 3.

ThermomiR expression increases at 40°C in THP-1-derived macrophages. (A) Expression of temperature-sensitive miRNAs (thermomiRs) measured by small RNA sequencing at 37°C and after 2, 8 and 24 h of incubation at 40°C. (B) Expression of thermomiRs and controls measured by RT-qPCR after incubation for 24 h. Data are from three independent experiments each in triplicate and show mean ± SEM. Two-tailed Student's t-test was used to determine significance, denoted by * (P < 0.05) and ** (P < 0.001). ns, not significant.