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. 2016 Jan 28;44(6):2888–2897. doi: 10.1093/nar/gkw041

Figure 4.

Figure 4.

RBM3 siRNA knockdown increases the expression of thermomiRs. (A) Expression of RBM3 mRNA by RT-qPCR, and (B) RBM3 protein levels by Western blot in THP-1-derived macrophages nucleofected with negative control siRNA and each of the three siRNAs against RBM3: #58, #59 and #60. (C) Average counts of individual miRNAs from the three RBM3 knockdown experiments as measured using NanoString nCounter. Two miRNAs miR-142 and miR-143 showed the greatest change in expression. (D) Expression of pyrogens in THP-1-derived macrophages: IL6ST, TNF and TLR-2 following inhibition of miR-142–5p and miR-143 (relative to control inhibitor). (E) Expression of miR-142 and miR-143 in THP-1-derived macrophage at 24 h after nucleofection with respective miRNA mimics (relative to control). (F) Expression of IL6ST, TNF and TLR- in THP-1-derived macrophage at 24 h following nucleofection of miR-142 and miR-143 mimic (relative to control). Data are from three independent experiments each in triplicate and show mean ± SEM. Two-tailed Student's t-test was used to determine significance, denoted by * (P < 0.05) and ** (P < 0.001). inh, inhibitor; ns, not significant.