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. 2016 Feb 22;44(6):2795–2805. doi: 10.1093/nar/gkw097

Figure 4.

Figure 4.

Cs-TopIB supercoiled DNA relaxation assays over broad temperature range. (A) Effect of the temperature: assays were carried out for 2 min using 200 ng of pBR322 as a substrate of negative supercoiled DNA and 50 nM of Cs-TopIB protein at the indicated temperatures (°C). (B) Time courses assays were performed using 200 ng of pBR322 as a substrate of negative supercoiled DNA and 50 nM of Cs-TopIB protein at the indicated times (s). In both panel, Sc−: negatively supercoiled DNA; R: relaxed DNA; C: control reaction without enzyme.