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. 2016 Feb 24;44(6):2816–2826. doi: 10.1093/nar/gkw109

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Indenoisoquinoline-stabilized topo I-DNA covalent complexes persist for at least 4 h. (A) A549 cells grown on coverslips were treated with 1 μM indotecan for the indicated length of time, fixed and prepared for staining with the indicated antibodies. Cells were considered positive if they contained >10 foci. Error bars, ±sd of three independent experiments. (B–D) A549 cells treated with diluent or 1 μM indotecan were stained with α-TopoIcc and rabbit anti-γH2AX (B), anti-Rad51 (C) or anti-RPA70 (D) followed by fluorochrome-labeled secondary antibody and Hoechst 33258.