Table 4.
Effect of the dietary regime (TFD or C) and age (8 or 24 wk) on metabolite concentrations
| Diet |
Age |
Diet × Age |
||||
|---|---|---|---|---|---|---|
| Metabolite | F | Q | F | Q | F | Q |
| DAG (18:0_20:4) | 299.1* | <0.001* | 59.1* | <0.001* | 80.7* | <0.001* |
| Cer (d18:1/24:1) | 214.7* | <0.001* | 1.3 | 0.396 | 0.4 | 0.668 |
| DAG (18:1/18:1) | 183.1* | <0.001* | 28* | <0.001* | 35.7* | <0.001* |
| DAG (18:1_20:4) | 135.8* | <0.001* | 20.1 | 0.002* | 27.9* | <0.001* |
| Cer (d18:1/24:0) | 133.8* | <0.001* | 2.9 | 0.268 | 2.9 | 0.281 |
| DAG (18:1_18:2) | 110.5* | <0.001* | 9.1* | 0.039* | 11.7* | 0.023* |
| DAG (16:0_18:1) | 100.5* | <0.001* | 13.3* | 0.014* | 14.9* | 0.01* |
| Cer (d18:1/23:0) | 95.3* | <0.001* | 0.1 | 0.724 | 1.8 | 0.362 |
| DAG (16:1_22:6) | 75.9* | <0.001* | 6.1 | 0.091 | 7.2 | 0.072 |
| DAG (16:1_16:1) | 73.7* | <0.001* | 5.9 | 0.091 | 9.1* | 0.047* |
| Cer (d18:1/18:0) | 68.8* | <0.001* | 1.3 | 0.396 | 0.1 | 0.812 |
| DAG (18:2_20:4) | 68.8* | <0.001* | 9.0* | 0.039* | 21.6* | 0.002* |
| DAG (16:0_16:1) | 66.7* | <0.001* | 10.4* | 0.028* | 6.9 | 0.078 |
| DAG (34:2) | 65.8* | <0.001* | 5.1 | 0.119 | 7.8 | 0.071 |
| DAG (18:2/18:2) | 56.2* | <0.001* | 0.6 | 0.539 | 3.4 | 0.247 |
| DAG (16:1_18:2) | 54.5* | <0.001* | 3.8 | 0.204 | 3.0 | 0.281 |
| DAG (16:0_22:6) | 53.2* | <0.001* | 1.3 | 0.396 | 2.1 | 0.334 |
| Carnitine | 51.6* | <0.001* | 10.7 | 0.028 | 1.4 | 0.405 |
| Cer (d18:1/22:0) | 51.2* | <0.001* | 3.1 | 0.268 | 5.8 | 0.097 |
| DAG (18:1_22:6) | 49.3* | <0.001* | 1.3 | 0.396 | 6.1 | 0.094 |
| Alanine | 26.3* | <0.001* | 1.7 | 0.372 | 0.01 | 0.923 |
| Cer (d18:1/16:0) | 25.9* | <0.001* | 0.5 | 0.547 | 0.9 | 0.492 |
| Acetylcarnitine | 25.9* | <0.001* | 2.5 | 0.312 | 0.02 | 0.909 |
| DAG (20:2_20:4) | 24.5* | <0.001* | 1.8 | 0.372 | 6.7 | 0.078 |
| Succinate | 23.1* | <0.001* | 74.5* | <0.001* | 7.2 | 0.072 |
| DAG (16:0_18:0) | 22.5* | <0.001* | 1.2 | 0.421 | 1.3 | 0.405 |
| DAG (18:0_22:6) | 21.8* | <0.001* | 1.7 | 0.372 | 1.5 | 0.398 |
| DAG (22:6/22:6) | 19.4* | <0.001* | 1.3 | 0.396 | 0.3 | 0.675 |
| α-Ketoglutarate | 14.4* | 0.002* | 0.3 | 0.604 | 0.02 | 0.909 |
| Cer (d18:1/20:0) | 13.2* | 0.003* | 2.9 | 0.268 | 9.6 | 0.043 |
| DAG (18:2_20:2) | 12.1* | 0.004* | 1.3 | 0.396 | 3.8 | 0.224 |
| n-Butyrylcarnitine | 10.7* | 0.006* | 0.4 | 0.59 | 0.07 | 0.857 |
| Isovalerylcarnitine | 8.7* | 0.012* | 4.4 | 0.157 | 1.8 | 0.362 |
| Glutamate | 6.0* | 0.038* | 5.3 | 0.114 | 1.5 | 0.398 |
DAG, diacylglycerol; Cer, ceramide.
F and Q are F-statistics and false discovery rate-corrected P values, respectively, from linear models (see text). Metabolites are ranked by statistical significance of the effect of diet.
*
Significant effects.