Table 3.
Some of the significantly induced transcripts in A549 cells by treatment with poultry dust extract
| Gene Alias | Gene Name | Fold change (1 h) | P Value | Fold Change (6 h) | P Value | Fold Change by qRT-PCR (6 h) |
|---|---|---|---|---|---|---|
| IL-6 | interleukin-6 | 62.9 | 1.85E-11 | 19.32 | 2.52E-07 | 59.8 ± 18.2 (P < 0.01) |
| IL-8 | inteleukin-8 | 35.75 | 4.06E-07 | 14.37 | 3.03E-11 | 42 ± 5.9 (P < 0.0001) |
| IL-11 | interleukin-11 | 2.92 | 0.00017 | 2.14 | 0.00095 | |
| IL-1β | interleukin-1β | ND | ND | 15.6 ± 3.98 (P < 0.01) | ||
| CCL2 | chemokine (C-C motif) ligand 2 | 6.3 | 6.10E-08 | 6.3 | 4.96E-09 | 9.2 ± 2.5 (P < 0.01) |
| CCL5 | 5.9 | 2.94E-05 | 17.4 ± 6.4 (P < 0.05) | |||
| CCL20 | chemokine (C-C motif) ligand 20 | 154.83 | 5.36E-13 | 80.42 | 1.92E-09 | |
| CXCL1 | chemokine (C-X-C motif) ligand 1 | 9.45 | 6.16E-09 | 8 | 9.44E-07 | |
| CXCL2 | chemokine (C-X-C motif) ligand 2 | 27.84 | 1.48E-08 | 7.64 | 3.03E-10 | |
| CXCL5 | chemokine (C-X-C motif) ligand 5 | 2.03 | 0.0028 | 5.23 | 3.73E-11 | |
| Cyr61 | cysteine rich angiogenic inducer 61 | 7.66 | 9.89E-09 | ND | 2.1 ± 0.3 (P < 0.05) | |
| ICAM1 | intercellular adhesion molecule 1 | ND | ND | 34.2 ± 10.3 (P < 0.01) | ||
| TLR4 | toll-like receptor 4 | ND | ND | 4.1 ± 0.66 (P < 0.001) | ||
| SOD2 | superoxide dismutase 2 | ND | 4.59 | 0.00016 | 2 ± 0.6 (P < 0.05) | |
| PTGS2 | prostaglandin-endoperoxidase synthase 2 | 5.14 | 3.94E-05 | 1.89 | 0.0069 | 4.5 ± 0.78 (P < 0.001) |
| EGR1 | early growth response protein 1 | 25.92 | 7.21E-14 | ND | ||
| JUN | v-Jun avian sarcoma virus 17 oncogene homolog | 4.64 | 1.24E-06 | 0.47 | 0.023 | |
| ETS1 | v-Ets avian erythroblastosis virus E26 oncogene homolog 1 | 4.86 | 0.00060 | ND | ||
| FOSB | FBJ murine osteosarcoma viral oncogene homolog B | 30852 | 7.34E-13 | ND | ||
| ATF3 | activating transcription factor 3 | 29.67 | 1.73E-12 | ND |
Changes (means ± SE, n = 4–7) in the levels of transcripts induced by dust extract, relative to untreated cells, determined by qRT-PCR are also shown. Genes listed include cytokines, chemokines, and transcription factors and proteins implicated in inflammatory responses. ND, not determined.