Fig. 4.

TUG-891 stimulation enhances GPR120-β-arrestin-2 interaction in Caco-2, but not in STC-1 cells. Top: lysates of control or 10 μM TUG-891-treated Caco-2 (A) or STC-1 (B) cells, containing equal amounts of proteins, were used to IP GPR120 with anti-β-arrestin-2 antibody. IPs were subjected to SDS-PAGE and probed with anti-GPR120 antibody in immunoblotting. After being stripped with 0.2 N NaOH, blots were reprobed with anti-β-arrestin-2 antibody. Representative blots of 3 independent experiments are shown. Bottom: densitometric analysis of band intensities of GPR120/β-arrestin-2 in different groups is shown. Values are means ± SE; n = 3. *Different from control, P ≤ 0.05.