Fig. 6.

Agonist stimulation of GPR120 inhibits NF-κB activation in Caco-2 and not in STC-1 cells. A: Caco-2 cells transfected with p-NF-κB-Luc reporter plasmid and β-galactosidase expression vector were pretreated with TNF-α (10 ng/ml) for 6 h, followed by treatments with GW9508 (50 μM) or TUG-891 (10 μM) in presence or absence of TNF-α (10 ng/ml) for another 24 h. Cells were harvested; luciferase activities were measured and normalized with the respective β-galactosidase activities. Values are means ± SE; n = 3. *Different from control, P ≤ 0.05. B, top: nuclear extracts were prepared from control or agonist (50 μM GW9508 or 10 μM TUG-891)-treated (6 h) STC-1 cells. Nuclear extracts were subjected to SDS-PAGE and probed with anti-p-65 antibody in immunoblotting. After being stripped with 0.2 N NaOH, blots were reprobed with anti-lamin antibody as loading control. Densitometric analysis of band intensities of p65/lamin in different groups is shown. Values are means ± SE; n = 3.