Fig. 7.

β-Arrestin-2 knockdown abrogates agonist stimulation-dependent internalization of GPR120 and inhibition of NF-κB. Caco-2 cells were transfected with control-scrambled small interfering (siRNA) (Si) or β-arrestin-2 siRNA, as described in materials and methods. Forty-eight hours after transfection, cells were used either to measure β-arrestin-2 levels by immunoblotting with anti-β-arrestin-2 antibody (A), or to measure surface GPR120 by cell surface biotinylation (B), or to prepare nuclear extracts and measure p65 levels by WB (C). Representative blots of 3 independent experiments are shown in A–C.