Fig. 2.

Unrestrained whole body plethysmography for the short-term treatment experiment. After LPS/elastase model induction and treatment with iloprost or saline placebo, Penh was measured in response to increasing doses of methacholine (MeCh). Penh was significantly higher in the LPS/elastase-exposed mice compared with wild-type, unexposed controls (WT-CTRL). There was no difference in Penh between the iloprost and saline groups after either one dose (A) or three doses (B). Comparisons were made using two-way ANOVA with repeated measures and Bonferroni's post hoc test. n = 6 for WT-CTRL, n = 8 for LPS/elastase + saline, and n = 8 for LPS/elastase + iloprost. *P ≤ 0.05 for LPS/elastase + iloprost vs. WT-CTRL; +P ≤ 0.05 for LPS/elastase + saline vs. WT-CTRL.