SsnB attenuates TLR ligand-induced macrophage activation, gene expression of P47phox, and gene expression and release of inflammatory cytokines in mouse primary hepatic macrophages. A: qRT-PCR analysis of mRNA expression of IL-1β, MCP1, and TNF-α from control (untreated), LPS-treated, and LPS+SsnB-treated mouse primary hepatic macrophages, normalized against control (*P < 0.05). B: TNF-α cytokine levels measured by ELISA from the supernatant collected from control, LPS-treated, and LPS+SsnB-treated mouse primary hepatic macrophages, normalized against control (*P < 0.05). C: qRT-PCR analysis of mRNA expression of p47phox from control (untreated), LPS-treated, and LPS+SsnB-treated mouse primary hepatic macrophages, normalized against control (*P < 0.05).