Figure 2.

Drug conjugation and characterization of activatable DOX (ND₂^DOX) and PXL (ND₂^PXL). A: Schematic representation of the synthetic pathway from ND₂^(Fmoc-Pep) (4) to either ND₂^DOX (6) or ND₂^PXL (7). B: SEC of ND₂^(Fmoc-Pep) (solid, left y-axis) and ND₂^Pep (dotted, right y-axis)). C: SEC of DOX (dotted) and ND₂^DOX (solid). D: SEC of ND₂^Pep (dotted, left y-axis) and of the reaction mix to link PXL to ND₂^Pep (solid, right y-axis).