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. Author manuscript; available in PMC: 2016 Apr 7.
Published in final edited form as: Methods Mol Biol. 2015;1279:25–43. doi: 10.1007/978-1-4939-2398-4_3

Table 1.

Primers necessary for checking for parts of the mutagenesis vector in individual mutant strains, nested semi-random PCR, and Tn-seq. The ermG and bla primers are for the genes of pGERM (EF155418.1) and the himar1c9a primers are for the gene of pBADC9 (PMCID: PMC18050).

Primer Sequence Function
ermG + TAGGTGCAGGGAAAGGTCAT Vector
ermG − CCATTTTTGCTGGCTTTCTT Vector
bla + TTGCCGGGAAGCTAGAGTAA Vector
bla − GCTATGTGGCGGTATTAT Vector
himar1c9a + GACGGAAAAACTCGGGTGTA Vector
himar1c9a − TTCAAGCGTGGTGAAATGAG Vector
SAMseq1 ACGTACTCATGGTTCATCCCGATA Semi-random
SAMseq2 GCGTATCGGTCTGTATATCAGCAA Semi-random
SAMseq3 TCTATTCTCATCTTTCTGAGTCCAC Semi-random
ARB1 GGCCACGCGTGCACTAGTACN10TACNG Semi-random
ARB2 GGCCACGCGTGCACTAGTAC Semi-random
Olj376 GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGGGGGGGGGGGGGGGG Illumina
pSAM1 CCTGACGGATGGCCTTTTTGCGTTTCTACC Illumina
pSAM2 AATGATACGGCGACCACCGAGATCTACACTCTTTGACCGGGGACTTATCATCCAACCTGTTA Illumina
pSAM3 ACACTCTTTGACCGGGGACTTATCATCCAACCTGTTA Illumina