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. Author manuscript; available in PMC: 2016 Apr 7.
Published in final edited form as: Biochim Biophys Acta. 2015 Dec 28;1859(2):393–404. doi: 10.1016/j.bbagrm.2015.12.008

Figure 2.

Figure 2

OsPIF14 binds to both OsDREB1B promoter N-box (N-box 1 and 2). A, Schematic representation of the eighteen probes used in Electrophoretic Mobility Shift Assay (EMSA) to screen OsDREB1B promoter. Each probe has 30 bases length in which 15 bases overlap with next probe. N-box 1 and 2 squares represent the sequence (CACGCG). Probes 2.7, 2.8 and 2.11 have intact N-box, while 2.12 lacks the first base of N-box, as represented by dotted lines. Probe 2.7 and 2.8 share the same N-box. B and C, EMSA results of OsDREB1B promoter probes screen showing that OsPIF14 binds to both N-box of OsDREB1B promoter with different intensities. FP, free probe. D, alignment of the three probes to which OsPIF14 binds in EMSA. Square represents the important bases for OsPIF14-DNA binding. Dark shaded bases represent the N-box. Light shaded bases represents the common bases between the three probes.