Table 2.
Summary of ANOVA on the effect of temperature regimes on transmission potential of Aedes aegypti for DENV-3 and mosquito survival
| All temperature regimes | 25°C ± 0°C vs. 25°C ± 4°C | 25°C ± 4°C vs. 28°C ± 4°C | |
|---|---|---|---|
| P value | Adjusted P value | Adjusted P value | |
| Proportion of infective mosquitoes | |||
| Temperature | ** | *** | 0.08 |
| Wolbachia | **** | *** | *** |
| Temperature × Wolbachia | 0.3 | 0.18 | 0.18 |
| EIP | |||
| Temperature | 0.71 | 0.52 | 0.46 |
| Wolbachia | 0.13 | 0.26 | 0.52 |
| Temperature × Wolbachia | 0.37 | 0.52 | 0.52 |
| Frequency of DENV detected in saliva | |||
| Temperature | 0.54 | 0.49 | 0.49 |
| Wolbachia | *** | ** | ** |
| Temperature × Wolbachia | 0.93 | 0.74 | 0.74 |
| Survival | |||
| WT | NA | 0.57 | 0.24 |
| wMel.F | NA | 0.24 | 0.32 |
ANOVA = analysis of variance; DENV = dengue virus; DENV-3 = dengue virus serotype 3; EIP = extrinsic incubation period; NA = not applicable; wMel.F = wMel-infected. P values for post hoc pairwise comparison statistics (between two temperature regimes) were corrected using the Benjamini and Hochberg method.51 *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.