Table 4.

Complete test results for 33 reference CSF classified as JE+ or DEN (JE−) at CDC

Sample number	CDC JEV P/N	CDC DENV P/N	JEV PRNT	DENV PRNT	CDC final interpretation*	JE Detect ISR	JE Detect results†	DEN Detect ISR	DEN Detect results‡	Inbios final interpretation
1	18.93	1.23	1:640	1:160	JE+	64.22	JE	2.20	EQ	JE+
2	20.08	1.57	1:4	< 1:4	JE+	44.83	JE	1.50	NEG	JE+
3	17.66	1.67	1:4	< 1:4	JE+	46.83	JE	1.15	NEG	JE+
4	11.93	1.80	1:8	< 1:8	JE+	67.15	JE	1.23	NEG	JE+
5	19.4	3.83	1:640	1:160	JE+	75.84	JE	3.3	DEN+	DEN+
6	19.07	3.30	1:8	< 1:4	JE+	83.81	JE	2.25	EQ	JE+
7	20.80	3.53	1:4	< 1:4	JE+	4.68 (4.1)	EQ	1.108	NEG	NEG
8	19.60	10.76	1:8	< 1:8	JE+	52.70	JE	6.14	DEN+	DEN+
9	31.02	5.41	1:8	< 1:8	JE+	86.17	JE	3.20	DEN+	DEN+
10	22.25	4.64	1:160	1:40	JE+	79.30	JE	2.54	EQ	JE+
11	24.11	9.17	1:320	1:80	JE+	74.34	JE	3.37	DEN+	DEN+
12	22.64	7.64	1:16	< 1:4	JE+	79.33	JE	3.6	DEN+	DEN+
13	18.87	6.91	1:16	< 1:4	JE+	68.67	JE	2.3 (2.14)	EQ	JE+
14	20.68	1.95	1:8	< 1:4	JE+	73.40	JE	1.36	NEG	JE+
15	21.68	3.10	1:4	< 1:4	JE+	79.58	JE	1.53	NEG	JE+
16	27.3	6.16	1:16	< 1:4	JE+	85.45	JE	3.76	DEN+	DEN+
17	10.87	14.52	1:320	< 1:20	JE+	84.29	JE	3.34	DEN+	DEN+
18	29.42	7.40	1:160	< 1:20	JE+	87.49	JE	2.8 (4.15)	EQ/DEN+	DEN+
19	6.72	1.20	1:320	< 1:20	JE+	82.74	JE+	1.35	NEG	JE+
20	6.41	1.77	1:320	1:20	JE+	48.05	JE	0.96	NEG	JE+
21	4.91	1.98	1:320	< 1:20	JE+	39.63	JE	1.26	NEG	JE+
22	3.56	1.68	1:80	< 1:20	JE+	6.86	JE	2.22 (2.51)	EQ	JE+
23	3.89	1.00	1:4	< 1:4	JE+	27.37	JE	1.07	NEG	JE+
24	3.99	0.94	1:8	< 1:8	JE+	53.11	JE	1.08	NEG	JE+
25	1.58	2.02	1:40	1:320	DEN+	4.57 (4.63)	EQ	1.03	NEG	NEG
26	1.44	2.01	< 1:2	1:320	DEN+	2.40	NEG	1.02	NEG	NEG
27	1.24	3.52	< 1:8	1:8	DEN+	2.97	NEG	1.44	NEG	NEG
28	1.799	3.92	< 1:4	1:4	DEN+	1.93	NEG	1.02	NEG	NEG
29	4.08	5.57	< 1:4	> 1:128	DEN+	3.84	NEG	1.52	NEG	NEG
30	2.84	2.15	< 1:4	1:32	DEN+	3.71	NEG	1.03	NEG	NEG
31	1.02	3.00	< 1:8	1:8	DEN+	4.8 (2.76)	EQ/NEG	2.79 (2.24)	EQ	NEG
32	2.32	3.46	< 1:4	1:4	DEN+	2.38	NEG	1.14	NEG	NEG
33	1.50	2.96	< 1:4	1:4	DEN+	2.05	NEG	1.16	NEG	NEG

CDC = Centers for Disease Control and Prevention; CSF = cerebrospinal fluid; DEN = dengue; DEN Detect = DEN Detect^™ MAC-ELISA; DENV = dengue virus; EQ = equivocal; ISR = Inbios Detect immune status ratio; JE = Japanese encephalitis; JE Detect = JE Detect^™ MAC-ELISA; JEV = Japanese encephalitis virus; MAC-ELISA = IgM antibody-capture enzyme-linked immunosorbent assay; NEG = negative; P/N = mean OD of sample reacted on viral antigen/mean OD of negative control sample reacted on viral antigen.

^*CDC ELISA interpretations: NEG P/N < 2; EQ P/N 2–3; POS, P/N > 3.

^†Calculation of JE Detect results: NEG, ISR < 4.0; EQ, ISR 4–6; JE+, ISR > 6.0.

^‡Calculation of DEN Detect results: NEG, ISR < 1.65; EQ, ISR 1.65–2.84; DEN+, ISR > 2.84.