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. 2016 Apr 6;94(4):890–893. doi: 10.4269/ajtmh.15-0566

Figure 2.

Figure 2.

Differential transcript abundance of TbLPPs. (A) Reverse transcriptase polymerase chain reaction (RT-PCR) using gene-specific primers in a 35-cycle PCR. The TbLPPs were normalized to tubulin (tub) (mean ± standard error [SE], from three independent biological samples; Supplemental Table 1). BSF, MCF, SG, cardia, and MG from 42-day-old infected tsetse flies. (B) Differential transcript abundance of TbLPPs in procyclic parasites from different phases of cellular growth. RT-PCR using gene specific primers in a 35-cycle PCR from parasites from lag, low log, high log, and stationary phases (mean ± SE, from three independent biological samples). BSF, MCF, SG, cardia, and MG from 42-day-old infected tsetse flies. (A and B) Statistical analyses are shown in Supplemental Tables 2 and 3. BSF = bloodstream form; MCF = metacyclic form; SG = salivary glands; MG = midgut from 42-day-old infected tsetse flies.