Figure 1.
Box plot of melt curve analysis–derived melting temperatures observed in individual real-time polymerase chain reaction with melt curve analysis reactions illustrating limited variation in Tm observed in reactions containing Brugia malayi (BM, N = 156), Dirofilaria immitis (DI, N = 79), and Onchocerca volvulus (OV, N = 82) positive controls and MF-positive skin snips (Snip, N = 509) from Uganda and Ethiopia. Box boundaries indicate one standard deviation and whiskers delineate minimum and maximum observed Tm.