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. Author manuscript; available in PMC: 2016 Apr 7.
Published in final edited form as: Int J Cancer. 2014 Oct 30;136(9):2055–2064. doi: 10.1002/ijc.29269

Figure 3.

Figure 3

Effect of JQ1 on expression of c-MYC, p21 and SIRT1 in human OS cells. Cells were treated with either DMSO (control) or 7.5 µM JQ1 subjected to qRT-PCR (6 hr) and Western blot analyses (12 hr). (a) mRNA expression level of c-MYC and p21 genes in JQ1-treated cells. Dotted lines indicate the normalized expression level of each gene in DMSO-treated control cells. GAPDH was used as a loading control. Measurements were repeated in triplicates. Data represent the mean relative expression ± standard deviation (SD; error bars). Asterisks (*) indicate p-value less than 0.05 (*) or 0.001 (**) by t-test. (b) Representative images showing changes in protein level of c-MYC, SIRT1 and p21 in JQ1-treated cells. Numbers indicate relative band intensity of each gene normalized to the intensity of the genes in control (1.0). GAPDH was used as a loading control.