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. 2016 Apr 7;11(4):e0153137. doi: 10.1371/journal.pone.0153137

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© 2016 Moen et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Images of the disk can be used for direct cell enumeration. A TIFF format picture was taken of each FOV using a Nikon TI inverted microscope (1,800 FOVs/sample lane. This photo shows one such FOV in section E, where the predominant visualized cell type is the leukocyte. Inset shows a monocyte that was removed from a disk post- centrifugation and cultured until a macrophage phenotype was seen. Images taken on Nikon TI inverted microscope (40X DIC) and auto white balanced using the NIS Elements AR 4.12.01 software.