(A) Serum levels of HBs in AAV/HBV carrier mice were monitored by ELISA after vaccination with EnxB. HBs values are presented as a nanogram per milliliter and are expressed as means ± SD of three mice in each time point. (B) Ayw subtype-specific anti-HBs antibody responses in AAV/HBV carrier mice were monitored by ELISA at indicated time points (n=3). (C) Serum HBs in HDI/HBV mice after EnxB- vaccination was monitored by ELISA (n=4). (D) Anti-HBs antibody responses in HDI/HBV mice were monitored by ELISA at indicated time points post vaccination (n=4). The arrows indicate the time points of EnxB vaccination. High, high antigenemia; Low, low antigenemia; NTC, no treatment control; EnxB, s.c. vaccinated with EngerixB; ND, not detected; NS, not significant; *P < 0.05, **P < 0.01, and ***P < 0.001versus corresponding control mice (throughout all figures). The data presented are representative of three independent experiments.