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. 2016 Apr 7;11(4):e0153290. doi: 10.1371/journal.pone.0153290

Fig 6. Inhibition of cell proliferation of Mv1Lu cells by GARP and TGFβ.

Fig 6

Bar diagrams show the proliferation rates (percentage of proliferation, n = 6) of treated versus untreated (100%) Mv1Lu cells. Cell numbers were determined using methylene blue staining of DNA [25]. (A) 2.5 x 104 cells were grown for 48h and treated with 200 ng/ml to 800 ng/ml of recombinant GARPTS to determine its influence on cell proliferation alone. (B) Cells were grown for 48h in the presence of the indicated TGFβ concentrations pre-incubated with or without the indicated GARPTS concentration. (C) Cells were incubated the same way like in (B), except that the proteins were pre-incubated in redox buffer to provoke covalent bonding of TGFβ and GARP (D) 60 ng/ml TGFβ were pre-incubated with different amounts of GARPTS (40–10 ng/ml) to determine the minimal amount of GARPTS needed to achieve maximal enhancement of TGFβ activation. The asterisk (*) marks a statistical significance p < 0.01.