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. Author manuscript; available in PMC: 2016 May 5.
Published in final edited form as: Nature. 2015 Oct 28;527(7576):105–109. doi: 10.1038/nature15548

Figure 1. LC3 interacts with nuclear lamina protein Lamin B1.

Figure 1

a, Proliferating young IMR90 cells were subjected to subcellular fractionation and immunoblotting. SE: short-exposure; LE: long-exposure. b, The nuclear fraction of IMR90 was pulled down with bacterially purified GST or GST-LC3B. c, GST-LC3B pulldown of purified Lamin B1 protein. d, Endogenous IP (immuno-precipitation) in IMR90. e, LC3 IP of IMR90 fractions. f, HEK293T transfected were subjected to GFP IP and immunoblotting. Bars are the mean ± s.e.m.; n=3; * P<0.001. ** P<0.0001; one-way ANOVA coupled with Tukey’s post hoc test (b); unpaired two-tailed Student’s t-test (f). Uncropped blots are in Supplementary Figure.