Fig 2. The activation function of CD48 and Ly9 depends on MHC class I recognition.

(A) Splenocytes from primed B6 and K^b D^b knock out mice were added to B16 cells stably transfected with CD48 or Ly9 before analyzing the production of IFNγ and the release of Lamp-1 by NK cells. Data are representative of 2 determinations. (B) Splenocytes from primed H-2^b mice (top row), D^d mice (middle row) D^d CD4-Cre mice (T cell-specific D^d deletion) (bottom row) were exposed to B16 cells expressing a control plasmid (B16) or B16 cells stably transfected with CD48 or Ly9 cDNA. Splenocytes were harvested and NK cells expressing Ly49A and lacking Ly49C, Ly49I and NKG2A (A+CIN-) were analyzed for their production of IFNγ and expression of cell surface of Lamp-1. (C, D) The bar graphs show mean percentage (±SEM) of IFNγ+, Lamp-1+ or IFNγ+ Lamp-1+ among A+CIN- NK cells following exposure to B16 cells (open bars) or B16 cells expressing CD48 (C) or Ly9 cDNA (D) (black bars) in 3 independent experiments using 1–2 mice in each experiment (total n = 3–6). Statistics: One-way ANOVA *p<0.05, **p<0.01, ***p<0.005, ns not significant (p>0.05).