Fig 4. Restoration of 2B4 function on NK cells.

(A) NK cells plus T cells (top row) or purified NK cells (bottom row) from naive B6, D^d and D^d CD4-Cre mice (T cell specific D^d deletion) were cultured in IL-2. After 5 days, cultures were exposed to B16 cells stably transfected with CD48. NK cells expressing Ly49A and lacking Ly49C, Ly49I and NKG2A (A+CIN-) were analyzed for the production of IFNγ and the expression of Lamp-1. (B) The bar graphs shows of A+CIN- NK cells from NK cell plus T cell cultures (open bars) or NK cell cultures (black bars) that are IFNγ+ Lamp-1+ following exposure to B16 cells stably transfected with CD48 (left) or to B16 cells expressing an empty control plasmid (right). Data depict means ± SEM from 2 independent experiments with 1 and 2 values per experiment (total n = 3). Statistics unpaired t-test *p<0.05, ns not significant (p>0.05).