Table 1. S. cerevisiae GFP-label detection in different developmental stages of Cx. pipiens by the identification of the presence of fluorescent signal observed under fluorescence microscopy, grown in YPDA culture medium and the amplification of the GFP gene by PCR.
| Fluorescence microscopy | Yeast GFP-label grown in YPDA medium | GFP gene amplification | |
|---|---|---|---|
| Developmental stage | Yeast detection | Yeast detection | GFP gene detection |
| Larvae 1 | +(20/20) | +(20/20) | +(17/20) |
| Larvae 2 | +(20/20) | +(20/20) | +(19/20) |
| Larvae 3 | +(20/20) | +(20/20) | +(15/20) |
| Larvae 4 | +(20/20) | +(20/20) | +(17/20) |
| Pupae | -(0/20) | +(14/20) | +(13/20) |
| Adult female | -(0/20) | -(0/20) | +(8/20) |
| Adult male | -(0/20) | -(0/20) | -(0/20) |
| Eggs F1 | -(0/10) | -(0/10) | -(0/10) |
| Larvae 1 F1 | -(0/20) | -(0/20) | -(0/20) |
(+): Positive detection of S. cerevisiae GFP-label. (-): Negative detection of S. cerevisiae GFP-label. In brackets: (positive detection of S. cerevisiae GFP-label / total analyzed).