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. 2016 Apr 7;11(4):e0153005. doi: 10.1371/journal.pone.0153005

Fig 2. The attenuation of NF-κB/p65 nuclear translocation by DYN 1–17 and the N-terminal fragments in LPS-stimulated THP-1 cells.

Fig 2

The LPS-stimulated THP-1 cells were treated with the N-terminal fragments of DYN 1–17 and U50,488H at 1 μM and 10 nM for an hour. The treated cells were fixed with paraformaldehyde (3.7%) and immunolabelled with primary anti-NF-κB/p65 monoclonal antibody and visualized using Alexa Fluor 555® secondary antibody. DAPI staining was used to identify the nuclei. The nuclear translocation of NF-κB/p65 in each treatment group was assessed using the Image Xpress screening system. Non-stimulated THP-1 cells (NS) served as negative control. The NF-κB/p65 translocation percentage in each treatment group was normalised and expressed relative to LPS-stimulated control group. Data shown are the means ± S.E.M. of at least three independent experiments performed in triplicates, *p ≤ 0.05.