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. 2016 Apr 7;11(4):e0153005. doi: 10.1371/journal.pone.0153005

Fig 3. Effect of IMD-0354 on the NF-κB/p65 nuclear translocation in LPS-stimulated THP-1 cells.

Fig 3

The LPS-stimulated THP-1 cells were treated with IMD-0354 (10 μM) and DMSO (0.1% v/v, as diluent control). The treated cells were fixed with paraformaldehyde (3.7%) and immunolabelled with primary anti-NF-κB/p65 rabbit monoclonal antibody and visualized using Alexa Fluor 555® secondary antibody. DAPI staining was used to identify the nuclei. The nuclear translocation of NF-κB/p65 in each treatment group was assessed using the Image Xpress system. Non-stimulated THP-1 cells (NS), receiving similar treatment served as negative control. The percentage of NF-κB/p65 nuclear translocation in each treatment group was normalised and expressed relative to LPS-stimulated control group. Data represents means ± S.E.M. of at least three independent experiments performed in triplicates, *p ≤ 0.05.