Fig 5. H. diminuta–infected IL-22^-/- display delayed and/or prolonged expression of immunoregulatory factors.

Wild-type (WT) and IL-22^-/- mice were infected with 5 H. diminuta and at time-points thereafter splenocytes (A) and mesenteric lymph node cells (MLN) (B) were excised and stimulated with conA (5 μg/ml) for 48 hr and IL-10 measured. (C) Shows increases in Foxp3 mRNA in the mid-jejunum of WT and IL-22^-/- post-infection. (D) Flow cytometry revealed increased numbers of CD4⁺Foxp3⁺ splenocytes 8 days post-infection (dpi) in both WT and IL-22^-/- mice (E, representative dot plots) (data are mean ± SEM; n = 6–9; * and # p<0.05 compared to strain control and time-matched WT, respectively).