Fig 9. Interleukin-25 is a major player in the anti-colitic effect in H. diminuta infected IL-22^-/- mice.

Interleukin-22^-/- mice were orally infected with 5 cysticercoids of H. diminuta and 8 days later received a single ip. injection of anti-IL-25 antibody (100 μg) concomitantly with delivery of DNBS (5 mg, ir.). Necropsy was performed 72 hr later and severity of colitis determined by (A) colon length, (B) macroscopic disease activity score, (C) histopathology scores, and conA stimulated splenocytes production of (D) IL-4 and (E) IL-10 (data are mean ± SEM; n = 6–8 from 2 independent experiments, except panel E where n = 3–5; * and δ p<0.05 compared to appropriate strain-matched control mice and wild-type (WT) DNBS, respectively) and # p<0.05 compared to animals infected and given anti-IL-25 blocking antibody.