Table 1. Strains and plasmids used in this study.
Strain | Relevant genotype | Source | ||
LT2 | Salmonella enterica serovar Typhimurium strain LT2 (wild type S. typhimurium LT2) | Salmonella Genetic Stock Center (SGSC) | ||
TSS500 | LT2 ΔcheY ΔcheZ | [17] | ||
TSS878 | LT2 Δtar ΔcheY ΔcheZ | [17] | ||
TSS868 | LT2 Δtsr ΔcheY ΔcheZ | [17] | ||
TSS866 | LT2 Δtar Δtsr ΔcheY ΔcheZ | [17] | ||
TSS941 | LT2 ΔmcpB ΔcheY ΔcheZ | This work | ||
TSS942 | LT2 ΔmcpC ΔcheY ΔcheZ | This work | ||
TSS958 | LT2 ΔmcpB ΔmcpC ΔcheY ΔcheZ | This work | ||
14028 | wild type S. typhimurium ATCC strain 14028 | Rasika Harshey [17] | ||
SM542 | 14028 ΔmcpB ΔmcpC | Rasika Harshey [17] | ||
TSS643 | LT2 Δaer ΔmcpC | Kelly Hughes | ||
Plasmid | Gene(s) | Resistance | Induction | Source |
pVS88 | cheZ-ecfp / cheY-eyfp | ampicillin | IPTG | [24] |
pKG116 | cloning vector | chloramphenicol | sodium salicylate | J.S. Parkinson |
pBR1 | mcpB | chloramphenicol | sodium salicylate | This work |
pML19 | mcpC | chloramphenicol | sodium salicylate | This work |