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. Author manuscript; available in PMC: 2017 Mar 14.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Feb 23;55(12):4097–4100. doi: 10.1002/anie.201600233

Figure 2.

Figure 2

Nanotriangle assembly and stability. Structures were analyzed by native PAGE in the presence of 2.5mM MgCl2. a) Top: Assembly of subdomain IIa short crystal construct containing single nucleotide (nt) overhangs (secondary structure shown in Figure 1a, top) and self-assembling small triangle construct containing four nucleotide overhangs (secondary structure shown in Figure 1c, top). Bottom: Assembly of subdomain IIa long crystal construct containing single nucleotide overhangs (secondary structure shown in Figure 1a, bottom) and self-assembling large triangle construct containing four nucleotide overhangs (panel c, top; secondary structure shown in Figure 1c, bottom). b) Stability of small triangle (top) and large triangle (bottom) when treated with or without 8M urea at room temperature (RT) or boiling. c) Diagram of large triangle (AAA, top) and programmable large triangle (ABC, bottom). d) Assembly of programmable large triangle (ABC) from corners A, B, and C.