Table 1. List of minicell types, bacterial strains, genotypes, and plasmids.

Minicell type	Strain	Genotype	Plasmid
lpxM+	VAX8I3	MG1655 (Δlac-pro, attBλ::proBAΩpTac::ftsZΩc1857-PR-PL::I-CeuI)	None
Vector (lpxM-)	VAX12B4	MG1655 (Δlac-pro, ΔlpxM, ΔdapA, attBλ::proBAΩpTac::ftsZΩc1857-PR-PL::I-CeuI)	None
VAX-I	VAX13G8	isogenic to VAX12B4	pVX-129 (inv+, pfo-)
VAX-P	VAX15D2	isogenic to VAX12B4	pVX-173 (inv-, pfo+)
VAX-IP	VAX14G8	isogenic to VAX12B4	pVX-156 (inv+, pfo+)