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. 2015 Aug 27;11(10):1891–1904. doi: 10.1080/15548627.2015.1085742

Figure 3.

Figure 3.

Sunitinib neutralized the lysosomal acidic pH and inhibited the proteolytic activity. (A) Acid pH-dependent fluorescence to LysoSensor Green DND-153 in 786-O cells in the absence or presence of sunitinib. (B) Quantification of the fluorescence shown in A (n = 3; ***, P < 0.001). (C) Quantification of the CTSB activity in 786-O cells in the absence (Ct) or presence of sunitinib (sun) in the organelles compartment (lysosomes, mitochondria, Golgi, endoplasmic reticulum) activity as described in Materials and Methods. (D) Analysis of the presence of pro and mature CTSB by immunoblotting. NAA10 is shown as a loading control.