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. 2015 Apr 20;11(10):1849–1863. doi: 10.1080/15548627.2015.1017185

Figure 5.

Figure 5.

Elaiophylin-induced cell death is partially apoptotic in ovarian cancer cells. (A) Effect of elaiophylin on the viability of ovarian cancer cell lines (OVCAR3, A2780, and SKOV3). Data are mean ± SEM of 3 experiments. (B) SKOV3 and A2780 cells were treated with elaiophylin for 24 h. Protein levels of cleaved CASP9, PARP1, BIRC5, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were detected by western blot. (C) SKOV3 and A2780 cells were treated with elaiophylin in the presence or absence of Z-VAD-FMK. PARP1 and GAPDH were detected by western blot. (D) Flow cytometric analysis of cell death. SKOV3, OVCAR3, and A2780 cells were treated for 24 h with elaiophylin (0.5 μM) or DMSO in the presence or absence of Z-VAD-FMK (25 μM). Data are means ± standard deviation of 3 independent experiments (*P < 0.05).