Skip to main content
. Author manuscript; available in PMC: 2016 Jul 4.
Published in final edited form as: Kidney Int. 2016 Jan 4;89(1):68–81. doi: 10.1038/ki.2015.293

Figure 1. Inhibition of Src with PP1 inhibits expression of fibronectin, α-SMA, and collagen 1 in cultured renal interstitial fibroblasts.

Figure 1

Cells were incubated in complete medium containing 5% FBS and were exposed to PP1 (1, 2, and 5 μM) for 24 h. Cell lysates were subject to immunoblot analysis with antibodies to Collagen 1, α-SMA, fibronectin, or α-Tubulin (a) and phospho-Src (Tyr416), Src and glyceroldehyde-3-phosphate dehydrogenase (GAPDH) (e). Representative immunoblots from three or more experiments are shown. Expression levels of indicated proteins were quantified by densitometry and normalized with α-Tubulin (b, c, d) or GAPDH (f). Data are represented as the mean ± SEM. Bars with different letters (a–d) are significantly different from one another (P<0.05).