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. Author manuscript; available in PMC: 2016 Jul 4.
Published in final edited form as: Kidney Int. 2016 Jan 4;89(1):68–81. doi: 10.1038/ki.2015.293

Figure 5. Src is activated in the kidney after UUO injury.

Figure 5

The left ureter was ligated for 3, 7, and 14 days. The kidneys were taken for immunoblot analysis of phospho-Src (Tyr-416) (p-Src), Src, GAPDH as indicated (a). Representative immunoblots from 3 experiments are shown. Expression levels of p-Src and Src were quantified by densitometry and normalized with GAPDH as indicated (b, c). Photomicrographs illustrate immunoflurecent staining of kidney tissue taken from the kidney subjected to UUO for 7 days with the antibodies to p-Src and α-SMA (600 x). The number of interstitial cells expressing p-Src, α-SMA, or p-Src + α-SMA were accounted respectively (d). Data are represented as the mean ± SEM (n=6). Bars with different letters (a–c) are significantly different from one another (P<0.05). Scale bar=20 μM.