Fig. 5.

Inhibition of candidate miRNAs down-regulates HbF in K562 cells. The effect of anti-miRNAs (5 mM) on HbF protein levels in K562 cells (western blot analysis—a; western blot densitometric illustration—b). Inhibition of miR-26b and miR-151-3p results in decreased HbF levels in comparison to the control anti-miRNA. HbF expression is partially recovered with HU treatment. All anti-miRNAs co-transfections result in reduced HbF levels, particularly co-transfection of anti-miR-26b and anti-miR-494 resulting in near-complete transient knock-down of HbF