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. 2016 Apr 8;7:458. doi: 10.3389/fpls.2016.00458

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Copyright © 2016 Aziz, Nadipalli, Xie, Sun, Surowiec, Zhang and Paré.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Glucosinolate accumulation in Arabidopsis by GB03. Representative UHPLC-PDA chromatogram showing shoot glucosinolate profile in both GB03- and water-treated 30-day-old plants (A); an asterisk (^∗) indicates peak alignment between the lower and upper chromatograms. GB03 induces total glucosinolate (GLS) accumulation in shoots (dark blue-GB03 versus light blue bar-H₂O control) and roots (dark green-GB03 versus light green bar-H₂O control) in 30-day-old plants (B). The aliphatic MSG and MTG as well as indolic glucosinolates are shown; an asterisk (^∗) indicates statistically significant difference between treatments (t-test, P-value ≤ 0.05, n = 6, mean ± SE). Internal standard, sinigrin; 4-MSOB, 4-methylsulfinylbutyl glucosinolate; 5-MSOP, 5-methylsulfinylpentyl glucosinolate; 6-MSOH, 6-methylsulfinylhexyl glucosinolate; 7-MSOH, 7-methylsulfinylheptyl glucosinolate; 4-MTB, 4-methylthiobutyl glucosinolate; 8-MSOO, 8-methylsulfinyloctyl glucosinolate; I3M, indol-3-ylmethyl glucosinolate; 4MOI3M, 4-methoxyindol-3-ylmethyl glucosinolate; 1MOI3M, 1-methoxyindol-3-ylmethyl glucosinolate; 7-MTH, 7-methylthioheptyl glucosinolate; and 8-MTO, 8-methylthiooctyl glucosinolate.