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. 2016 Apr 7;12:91. doi: 10.1007/s11306-016-1023-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — Extracted ion chromatograms of two isomeric lipid species obtained at 9.0 and 9.3 min (a). CID mass spectra of these two ions in the positive ionization mode shows the same fragmentation pattern, with the characteristic fragment of phosphocholine at m/z 184.0727. Additional CID spectra performed in the negative ionization mode enabled to determine the presence of the fatty acids FA 16:1 (m/z 253.2172) (b) and FA 16:0 (m/z 255.2327) (c), corresponding to two distinct PC species, namely alkyl–acyl-PC 32e:1 and alkenyl–acyl-PC32p:0