Functional tests of proteins for a role in promoting susceptibility to R. solani.
A, Photos of agroinfiltrated N. benthamiana leaves expressing recombinant proteins and subsequently inoculated with R. solani. B, Lesion sizes on R. solani inoculated N. benthamiana leaves expressing recombinant proteins, dark gray bars. Light gray bars, necrotic lesion on agroinfiltrated leaves without R. solani inoculation. The average and standard error of four replicates are shown. Columns not connected by same letter are significantly different according to Student's t test. With the exception of NLP1, all leaves not inoculated with R. solani has a lesion size of 0. The experiment was repeated three times with similar results. Thaumatin, RsAG8_08836; GFP and DsRed, fluorescent reporter genes used as controls; NLP1, Necrosis and ethylene inducing protein like protein from F. oxysporum 5176 (FOXB_01740); Endotoxin 1, RSAG8G_05753; Endotoxin 2, RSAG8G_06697; RsRCN1, RSAG8G_10097; RsRCN2, RsAG8_02872.