Fig. 2.

FA96A is not involved in the cellular response to brusatol. (A) Melting curve of FA96A from lysates of cells treated with DMSO or 500 nm brusatol for 30 min. Green: DMSO treatment; red: brusatol treatment. (B) A549 lysates were treated with 500 nm brusatol for 30 min and soluble proteins from the indicated temperatures fractionated by SDS-PAGE and Western blotted using the indicated antibodies. *denotes nonspecific bands. (C) A549 cells were treated with either nontargeting (42) or siRNA oligos targeting FA96A for 3 days. Lysates were then treated with the indicated amounts of brusatol for 30 min, and indicated proteins detected by Western blotting.